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Published Work


Gang SS, Grover M, Reddy KC, Raman D, Chang YT, Ekiert DC, Barkoulas M, Troemel ER. A pals-25 gain-of-function allele triggers systemic resistance against natural pathogens of C. elegans.PLoS Genet. 2022 Oct 3;18(10):e1010314. 

Katsanos D. and Barkoulas M. Targeted DamID in C. elegans reveals a direct role for LIN-22 and NHR-25 in antagonizing the epidermal stem cell fate. Sci Adv. 2022 Feb 4;8(5):eabk3141. 

eb 4;8(5):eabk3141. 


Katsanos D, Ferrando-Marco M, Razzaq I, Aughey G, Southall TD, Barkoulas M. Gene expression profiling of epidermal cell types in C. elegans using Targeted DamID. Development 2021 Sep 1;148(17):dev199452. 


Grover M, Fasseas M, Essmann C, Kenneth L, Braendle C, Felix M-A, Glockling S, Barkoulas M. Infection of C. elegans by Haptoglossa species reveals shared features in the host response to oomycete detection. Frontiers in Cellular and Infection Microbiology 2021, 14;11:733094

Hintze M, Katsanos D, Shahrezaei V, Barkoulas M. Phenotypic Robustness of Epidermal Stem Cell Number in C. elegans Is Modulated by the Activity of the Conserved N-acetyltransferase nath-10/NAT10. Front Cell Dev Biol. 2021 May 18;9:640856.


Koneru SL, Hintze M, Katsanos D, Barkoulas M. Cryptic genetic variation in a heat shock protein modifies the outcome of a mutation affecting epidermal stem cell development in C. elegans. Nature Communications. 2021 May 31;12(1):3263. 

Koneru SL, Quah FX, Ghose R, Hintze M, Gritti N, van Zon JS, Barkoulas M. A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans. Sci Rep. 2021 May 7;11(1):9787. 

Kalita R, Flanagan W, Lightley J, Kumar S, Alexandrov Y, Garcia E, Hintze M, Barkoulas M, Dunsby C, French PMW. Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast. J Biophotonics. 2021 Dec;14(12):e202100144. 


Grover M and Barkoulas M. C. elegans as a new tractable host to study infections by animal pathogenic oomycetes.PLoS Pathog. 2021 Mar 4;17(3):e1009316. 


Fasseas MK, Grover M, Drury F, Essmann CL, Kaulich E, Schafer WR, Barkoulas M. Chemosensory Neurons Modulate the Response to Oomycete Recognition in Caenorhabditis elegans. Cell Rep. 2021 Jan 12;34(2):108604. In simple words: 


Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, Spencer SL, Martin BL, Matus DQ. Visualizing the metazoan proliferation-quiescence decision in vivo.  Elife 2020 Dec 22;9:e63265. 

Hintze M, Koneru SL, Gilbert SPR, Katsanos D, Lambert J and Barkoulas M. A cell fate switch in the C. elegans seam cell lineage occurs through modulation of the Wnt asymmetry pathway in response to temperature increase. Genetics 2020 April 1, 2020 vol. 214 no. 4 927-939. In simple words:


Reddy KC, Dror T, Underwood RS, Osman GA, Elder CR, Desjardins CA, Cuomo CA, Barkoulas M, Troemel ER. Antagonistic paralogs control a switch between growth and pathogen resistance in C. elegansPLoS Pathog. 2019 Jan 14;15(1):e1007528. 


 Osman GA, Fasseas MK, Koneru SL, Essmann CL, Kyrou K, Srinivasan MA, Zhang, Sarkies P, Félix M-A and Barkoulas M. Natural infection of C. elegans by an oomycete reveals a new pathogen-specific immune response. Curr Biol 2018, 28, 640–648


Katsanos D., Koneru S.L., Mestek Boukhibar L.,Gritti N., Ghose R., Appleford P.J, Doitsidou M., Woollard A., van Zon J.S , Poole R.J and Barkoulas M. (2017). Stochastic loss and gain of symmetric divisions in the C. elegans epidermis perturbs robustness of stem cell number. PLoS Biol Nov 6;15(11):e2002429


Barkoulas M*, Vargas Velazquez AM, Peluffo AE, Félix MA. Evolution of new cis-regulatory motifs required for cell-specific gene expression in Caenorhabditis. PLoS Genetics 2016 Sep 2;12(9):e1006278


Matus DQ, Lohmer LL, Kelley LC, Schindler AJ, Kohrman AQ, Barkoulas M, Zhang W, Chi Q, Sherwood DR. Invasive cell fate requires G1 cell-cycle arrest and histone deacetylase-mediated changes in gene expression. Dev Cell. 2015 Oct 26;35(2):162-74 

van Zon JS, Kienle S, Huelsz-Prince G, Barkoulas M, van Oudenaarden A.Cells change their sensitivity to an EGF morphogen gradient to control EGF-induced gene expression. Nat Commun 2015 May 11;6:7053. 

Félix MA, Barkoulas M. Pervasive robustness in biological systems.  Nat Rev Genet. 2015 Aug;16(8):483-96.

Mestek Boukhibar L, Barkoulas M. The developmental genetics of biological robustness.  Ann Bot. 2015Apr;117(5):699-707.

2014 and before

Hay AS, Pieper B, Cooke E, Mandáková T, Cartolano M, Tattersall AD, Ioio RD, McGowan SJ, Barkoulas M, Galinha C, Rast MI, Hofhuis H, Then C, Plieske J, Ganal M, Mott R, Martinez-Garcia JF, Carine MA, Scotland RW, Gan X, Filatov DA, Lysak MA, Tsiantis M. Cardamine hirsuta: a versatile genetic system for comparative studies. Plant Journal 2014 Apr;78(1):1-15.

Barkoulas M., van Zon J.S., Milloz J., van Oudenaarden A., and Félix M.-A. Robustness and epistasis in the C. elegans vulval signaling network revealed by pathway dosage modulation. Dev Cell 2013 Jan 14;24(1):64-75

Félix M.-A., Barkoulas M. Robustness and flexibility in nematode vulva development. Trends in Genetics 2012 28 (4): 185-195

Hoyos E., Kim K., Milloz J., Barkoulas M., Pénigault J-B., Munro E., Félix M-A. Quantitative variation in autocrine signaling and pathway crosstalk in the Caenorhabditis vulval network. Curr Biol 2011 21 (7): 527 – 538.

Bilsborough G.D.*, Runions A.*, Barkoulas M.*, Jenkins H.W, Hasson A., Galinha C., Laufs P., Hay A., Prusinkiewicz P., and Tsiantis M. Model for the regulation of Arabidopsis thalianaleaf margin development. PNAS 2011 108 (8): 3424-3429.

Prasad K., Grigg S.P*, Barkoulas M.*, Ram Kishor Yadav, Gabino F. Sanchez-Perez, Violaine Pinon, Blilou I., Hofhuis H., Dhonukshe P., Galinha C., Mähönen A.P, Muller W.H, RamanS., Verkleij A.J, SnelB., Reddy G.V, Tsiantis M., Scheres B. Arabidopsis PLETHORA transcription factors control phyllotaxis. Curr Biol 2011 21 (13): 1123-1128.

Scarpella E., Barkoulas M., Tsiantis M. Control of leaf and vein development by auxin. Cold Spring Harb Perspect Biol. 2010 2(1): a001511.

Canales C., Barkoulas M., Galinha C., Tsiantis M. Weeds of change: Cardamine hirsuta as a new model system for studying dissected leaf development. J Plant Res. 2010 123 (1): 25-33.

Barkoulas M., Hay A., Kougioumoutzi E. and Tsiantis M.A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsuta. Nature Genetics 2008 40(9): 1136-1141.


Barkoulas M., Galinha C., Grigg S.P., Tsiantis M. From genes to shape: regulatory interactions in leaf development. Curr Opin Plant Biol. 2007 10 (6): 660-6.


Hay A., Barkoulas M.* and Tsiantis M. ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis. Development2006 133 (20): 3955-3961.


Hay A., Barkoulas M., Tsiantis M. PINning down the connections: transcription factors and hormones in leaf morphogenesis. Curr Opin Plant Biol 2004 7 (5):575-81

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